Anopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species

Background: Mosquito saliva is a complex cocktail whose pharmacological properties play an essential role in blood feeding by counteracting host physiological response to tissue injury. Moreover, vector borne pathogens are transmitted to vertebrates and exposed to their immune system in the context of mosquito saliva which, in virtue of its immunomodulatory properties, can modify the local environment at the feeding site and eventually affect pathogen transmission. In addition, the host antibody response to salivary proteins may be used to assess human exposure to mosquito vectors. Even though the role of quite a few mosquito salivary proteins has been clarified in the last decade, we still completely ignore the physiological role of many of them as well as the extent of their involvement in the complex interactions taking place between the mosquito vectors, the pathogens they transmit and the vertebrate host. The recent release of the genomes of 16 Anopheles species offered the opportunity to get insights into function and evolution of salivary protein families in anopheline mosquitoes. Results: Orthologues of fifty three Anopheles gambiae salivary proteins were retrieved and annotated from 18 additional anopheline species belonging to the three subgenera Cellia, Anopheles, and Nyssorhynchus. Our analysis included 824 full-length salivary proteins from 24 different families and allowed the identification of 79 novel salivary genes and re-annotation of 379 wrong predictions. The comparative, structural and phylogenetic analyses yielded an unprecedented view of the anopheline salivary repertoires and of their evolution over 100 million years of anopheline radiation shedding light on mechanisms and evolutionary forces that contributed shaping the anopheline sialomes. Conclusions: We provide here a comprehensive description, classification and evolutionary overview of the main anopheline salivary protein families and identify two novel candidate markers of human exposure to malaria vectors worldwide. This anopheline sialome catalogue, which is easily accessible as hyperlinked spreadsheet, is expected to be useful to the vector biology community and to improve the capacity to gain a deeper understanding of mosquito salivary proteins facilitating their possible exploitation for epidemiological and/or pathogen-vector-host interaction studies

Antigeni salivari quali strumenti epidemiologici per la valutazione dell'esposizione umana ad Aedes albopictus

Hematophagous arthropods during feeding inject into their hosts a cocktail of salivary proteins whose main role is to allow for an effective blood meal by counteracting host hemostasis, inflammation and immunity. However, saliva of blood feeders also evokes in vertebrates an antibody response that can be used to evaluate exposure to disease vectors. Salivary transcriptome studies carried out in different hematophagous species in the last fifteen years clarified the complexity of the salivary repertoires of blood feeding arthropods, pointing out that salivary proteins evolve at a fast evolutionary rate and highlighting the existence of family-, genus- and sometime even species-specific salivary proteins. Focusing on mosquitoes of the genera Anopheles and Aedes, which are important vectors of the human malaria parasite Plasmodium falciparum and of several arboviruses, we summarize here recent efforts to exploit genus-specific salivary proteins as biomarkers of human exposure to these vectors of large relevance for public health

Towards Incremental Parsing of Natural Language using Recursive Neural Networks

In this paper we develop novel algorithmic ideas for building a natural language parser grounded upon the hypothesis of incrementality. Although widely accepted and experimentally supported under a cognitive perspective as a model of the human parser, the incrementality assumption has never been exploited for building automatic parsers of unconstrained real texts. The essentials of the hypothesis are that words are processed in a left-to-right fashion, and the syntactic structure is kept totally connected at each step. Our proposal relies on a machine learning technique for predicting the correctness of partial syntactic structures that are built during the parsing process. A recursive neural network architecture is employed for computing predictions after a training phase on examples drawn from a corpus of parsed sentences, the Penn Treebank. Our results indicate the viability of the approach andlay out the premises for a novel generation of algorithms for natural language processing which more closely model human parsing. These algorithms may prove very useful in the development of eÆcient parsers

Ontological domain coding for cultural heritage mediation

Abstract. An ontology-based representation of information about a domain is flexible enough to support different strategies in presenting the information. In this paper, we present two applications for creating interactive presentations in a cultural heritage domain, that share the same database of informative units and the same representation of the domain, encoded in a light-weight ontology. An application for drama-based guided tours assembles the informative units in a location-aware fashion, by exploiting the structure of the ontology to enforce the notion of discourse focusing in the generated presentation. A browsing-based application for accessing the informative units supports semantic search, consulting the ontology to suggest modifications of the user's search to circumscribe or enlarge the result sets

MicroRNAs from saliva of anopheline mosquitoes mimic human endogenous miRNAs and may contribute to vector-host-pathogen interactions

During blood feeding haematophagous arthropods inject into their hosts a cocktail of salivary proteins whose main role is to counteract host haemostasis, inflammation and immunity. However, animal body fluids are known to also carry miRNAs. To get insights into saliva and salivary gland miRNA repertoires of the African malaria vector Anopheles coluzzii we used small RNA-Seq and identified 214 miRNAs, including tissue-enriched, sex-biased and putative novel anopheline miRNAs. Noteworthy, miRNAs were asymmetrically distributed between saliva and salivary glands, suggesting that selected miRNAs may be preferentially directed toward mosquito saliva. The evolutionary conservation of a subset of saliva miRNAs in Anopheles and Aedes mosquitoes, and in the tick Ixodes ricinus, supports the idea of a non-random occurrence pointing to their possible physiological role in blood feeding by arthropods. Strikingly, eleven of the most abundant An. coluzzi saliva miRNAs mimicked human miRNAs. Prediction analysis and search for experimentally validated targets indicated that miRNAs from An. coluzzii saliva may act on host mRNAs involved in immune and inflammatory responses. Overall, this study raises the intriguing hypothesis that miRNAs injected into vertebrates with vector saliva may contribute to host manipulation with possible implication for vector-host interaction and pathogen transmission

Androgen receptor polymorphisms and testicular cancer risk

Testicular cancer (TC) is currently the most common malignant solid tumour in Caucasian males aged 15-39 years. Epidemiological evidence suggests that its onset may be due to an imbalance in the action of steroidal sex hormones and their receptors. A faulty androgen receptor signalling pathway can, in fact, cause various male reproductive disorders. The androgen receptor (AR) gene has two polymorphic segments consisting of CAG and GGC repeats. The length of CAG repeats has been shown to affect the regulation of AR activity. In our study, we used fragment analysis to evaluate the AR gene repeats of 302 TC patients and 322 controls, to establish if there is any association between repeat number and TC. This study of the largest Italian caseload investigated to date highlighted three particularly significant aspects. First, a CAG repeat number of ≥25 may be considered a risk factor for the onset of TC, given its greater frequency in patients in comparison with controls. This difference became significant for the non-seminoma group. Second, men with CAG repeats below 21 or above 24 were found to have a, respectively, 50 and 76% higher risk of TC than those with CAG 21-24, suggesting that these too can be considered a risk factor for TC. Finally, stage II patients were more likely to have a CAG repeat number 24 than stage I patients. © 2014 American Society of Andrology and European Academy of Andrology